Año: 2020
Autores: Aguirre Zapata, E.; MORALES, H.; di Sciascio, F.; Amicarelli, A.;
Resumen: Biotechnology is the area of science that aims to generate technology-based solutions through the use of living organisms. In this sense, in areas such as agriculture, applications such as the production of bioinsecticides have emerged that seek to replace the harmful use of chemical insecticides for the care of the environment and the health of the final consumers of the products. The bacterium Bacillus thuringiensis has become in recent years as one of the most widely used solutions for the production of δ -endotoxins that turn out to be harmful to some invertebrates such as larvae, which are present in numerous crops Considering the above, some research has focused on the knowledge of this bacterium and the development of mathematical models for different tasks such as control and estimation of variables. In this work, we present the design and simulation of a growth rate control for the production of δ -endotoxins, by implementing two coupled NMPC controllers for the control of µ growth rate and concentration of dissolved oxygen CDO. The proposed controller coupled with an Unscented Kalman Filter, allows to optimally control the growth rate of the cells, resulting in a significant increase in the concentration of total cells (biomass) in the reactor and therefore in a higher production of δ-endotoxins.